effects of different medium on morphophysiological traits of lippia citriodora h.b.k. during in vitro culture condition

نویسندگان

hassan nourafcan

fatemeh sefidkon

amir mousavi

mozaffar sharifi

ahmad khalighi

چکیده

background & aim: lemon verbena (lippia citriodora h.b.k.) is a richly scented herb and has a long history as a sacred and medicinal plant. select an appropriate medium in tissue culture is a necessity for success experimental: to evaluate the effect of 10 medium of previous researches for lippia micropropagation (1- ms medium supplemented with bap (3 mgl-1) + iba (0.1 mgl-1) + activated charcoal (1 gl-1) 2- ms medium supplemented with bap (1 mgl-1) + iba (0.5 mgl-1) + activated charcoal (1 gl-1) 3- 1/2ms 4- ms medium supplemented with bap (5 mgl-1) 5- ms medium supplemented with bap (1 mgl-1) 6- ms medium supplemented with activated charcoal (1 gl-1) 7- ms medium supplemented with bap (1 mgl-1) + activated charcoal (1 gl-1) 8- ms medium supplemented with bap (1 mgl-1) + agno3 (5 mgl-1) 9- ms medium supplemented with agno3 (5 mgl-1) 10- ms (control)), an experiment was conducted as a completely randomized design (crd) with three replicates and three explants per replication.  results & discussion: the results showed positive effects for bap (5 mgl-1) and somewhat active charcoal, but contrary to predictions, agno3 had no effect on reducing senescence and leaf necrosis. recommended applications/industries: therefore, based on the finding, the four medium (ms medium supplemented with bap (5 mgl-1)) with the highest rates of shoot fresh weight, leaf number, internodes number, shoot number, root fresh weight, callus diameter and root dry weight can be introduced as a suitable medium of tissue culture in this experimental conditions.

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Effects of different medium on morphophysiological traits of Lippia citriodora H.B.K. during in vitro culture condition

Background & Aim: Lemon verbena (Lippia citriodora H.B.K.) is a richly scented herb and has a long history as a sacred and medicinal plant. Select an appropriate medium in tissue culture is a necessity for success Experimental: To evaluate the effect of 10 medium of previous researches for Lippia micropropagation (1- MS medium supplemented with BAP (3 mgL-1) + IBA ...

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Effects of different medium on morphophysiological traits of Lippia citriodora H.B.K. during in vitro culture condition

Background & Aim: Lemon verbena (Lippia citriodora H.B.K.) is a richly scented herb and has a long history as a sacred and medicinal plant. Select an appropriate medium in tissue culture is a necessity for success Experimental: To evaluate the effect of 10 medium of previous researches for Lippia micropropagation (1- MS medium supplemented with BAP (3 mgL-1) + IBA ...

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the effect of e-64 on the developmental competence of sheep cocs during in vitro maturation

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Essential Oil Content and Composition of Lemon Verbena (Lippia citriodora Kunth.) during Different Phenological Stages

In this study, aerial parts of Lippia citriodora Kunth were harvested at three phenological stages (i.e. vegetative, full flowering and fruit set). The essential oil isolated by hydro distillation for 3 h using a Clevenger-type apparatus and the oil was analyzed by GC and GC-MS. The yields of oils (w/w%) in different stages was in the order of: full flowering (0.9%), vegetative (0.48%) and frui...

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عنوان ژورنال:
journal of herbal drugs (an international journal on medicinal herbs)

ناشر: research center for medicinal plants & ethno-veterinary, i.a.u., shahrekord branch

ISSN 2008-8884

دوره 5

شماره 3 Special issue (in Persian or Farsi) 2014

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